Genetic Diversity of Colletotrichum spp. an Endophytic Fungi in a Medicinal Plant, Brazilian Pepper Tree

نویسندگان

  • J. S. Lima
  • J. G. Figueiredo
  • R. G. Gomes
  • D. Stringari
  • E. H. Goulin
  • D. Adamoski
  • V. Kava-Cordeiro
  • L. V. Galli-Terasawa
  • C. Glienke
چکیده

In this study, we reported thirty-nine endophytic fungi identified as Colletotrichum spp. associated with Brazilian pepper tree or aroeira (Schinus terebinthifolius Raddi. Anacardiaceae) in Paraná state, Brazil. These endophytes were identified by morphological and molecular methods, using PCR taxon-specific with CaInt/ITS4, CgInt/ITS4, and Col1/ITS4 primers, which amplify specific bands in C. acutatum, C. gloeosporioides lato sensu, and Colletotrichum boninensis, respectively, and by DNA sequence analysis of the nrDNA internal transcribed spacer region (ITS1, 5.8S, ITS2). We also assayed the presence of dsRNA particles in Colletotrichum spp. isolates. Combining both morphological characters and molecular data, we identified the species C. gloeosporioides, C. boninense, and C. simmondsii. However, we found a high genetic variability intraspecific in C. gloeosporioides which suggests the existence of several other species. Bands of double-stranded RNA (dsRNA) were detected in three of thirty-nine isolates. Identity of these bands was confirmed by RNAse, DNAse, and S1 nuclease treatments for the isolates LGMF633, LGMF726, and LGMF729. This is the first study reporting these particles of dsRNA in C. gloeosporioides.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Diversity and Biological Activities of Endophytic Fungi Associated with Micropropagated Medicinal Plant Echinacea purpurea (L.) Moench

Echinacea is one of the top ten selling medicinal herbs in Europe and United States. Commercially available formulations may contain different plant parts of three species (Echinacea purpurea, E. pallida, and E. angustifolia). Our study evaluates the diversity of microbial community associated with healthy E. purpurea clones and their ability to produce defense compounds. We recovered and ident...

متن کامل

Biodiversity and tissue-recurrence of endophytic fungi in Tripterygium wilfordii

A total of 343 endophytic fungal isolates representing 60 taxa including 30 morphotypes were isolated from the different parts of the Chinese medicinal plant, Tripterygium wilfordii. In most cases fungal strains were only identified to genus because species identification was difficult in these speciose genera. Non-sporulating isolates were designated as Morphotypes 1 to 30. The endophytic asse...

متن کامل

Genetic diversity study of Ethiopian hot pepper cultivars (Capsicum spp.) using Inter Simple Sequence Repeat (ISSR) marker

Hot pepper (Capsicum spp.) is an economically important spice widely cultivated and consumed in Ethiopia. In spite of its wide importance, there is no information available on the molecular genetic diversity of this crop. Cultivars characterization is an important link between the conservation and utilization of plant genetic resources in various breeding programs. Using five ISSR prim...

متن کامل

Antimicrobial and antitumor activity and diversity of endophytic fungi from traditional Chinese medicinal plant Cephalotaxus hainanensis Li.

Endophytes from Cephalotaxus hainanensis Li, an important source of anti-leukemia drugs, have not been widely explored. In this study, 265 endophytic fungal isolates from C. hainanensis Li were screened for antimicrobial activities against tilapia, banana, rice, and rape and for antitumor activities against human leukemia cell lines (K562, NB4, and HL-60). Diversity was also analyzed. The resul...

متن کامل

Invitro Antibacterial Activity of Endophytic Fungal Extracts Isolated from a Pharmaceutically Important Plant Ficus Religiosa L. Kalyanaraman Rajagopal, Maheswari Sundharamoorthy, Arumugam P., Jasmith Basha W., Kathiravan Govindarajan and Radhai Rajendran

Ficus religiosa L is one of the important medicinal plant widely used in pharmaceutical research. It is widely distributed in India and a religiously important host. In the present study, antibacterial activity of endophytic fungi isolated from Ficus religiosa was carried out. Organic extract of five dominant endophytic fungi viz Botryodiplodia theobromae, Colletotrichum gloeosporioides, Phomop...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره 2012  شماره 

صفحات  -

تاریخ انتشار 2012